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	Provedor de dados Electron. J. Biotechnol. (2) Autor Yuan,Mei (2) Dang,Phat (1) Gong,Limin (1) Guo,Baozhu (1) He,Guohao (1) Li,Aiqin (1) Li,Shuangling (1) Meng,Ronghua (1) Wang,Xingjun (1) Xia,Han (1) Zhang,Yue (1) Zhao,Chuanzhi (1) Mais... Palavra-chave Arachis hypogaea L. (1) CDNA library (1) Chalcone isomerase (1) Cultivated peanut (1) Expression analysis (1) Microsatellites (1) Polymorphism (1) Simple sequence repeat (1) Mais... Tipo do documento Journal article (2) Ano 2012 (1) 2010 (1) País Chile (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Cloning and expression analysis of peanut (Arachis hypogaea L.) CHI gene Electron. J. Biotechnol. Zhang,Yue; Xia,Han; Yuan,Mei; Zhao,Chuanzhi; Li,Aiqin; Wang,Xingjun. Chalcone isomerase (CHI) is the key enzyme that catalyzes chalcone into (2S)-flavanol or (2S)-5-desoxidation flavanol. The full length cDNA (1050 bp) of AhCHI (Arachis hypogaea CHI gene) was cloned by large scale EST sequencing using a peanut immature seed cDNA library. Sequence analysis results indicated that it was a type I CHI gene (with the accession number JN660794). The ORF of AhCHI was 768 bp, encoding a peptide of 255 amino acids with a pI of 5.189. Sequence alignment showed that the coding region of AhCHI gene is highly conserved to compare with CHI genes from other plant species. Peanut cDNA microarray and semi-quantitative RT-PCR analysis indicated that AhCHI was highly expressed in pegs. The expression level in flower and root was higher than... Tipo: Journal article Palavras-chave: Arachis hypogaea L.; CDNA library; Chalcone isomerase; Expression analysis. Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100005 Development of trinucleotide (GGC)n SSR markers in peanut (Arachis hypogaea L.) Electron. J. Biotechnol. Yuan,Mei; Gong,Limin; Meng,Ronghua; Li,Shuangling; Dang,Phat; Guo,Baozhu; He,Guohao. Cultivated peanut (Arachis hypogaea L.) is an oilseed crop of economic importance. It is native to South America, and it is grown extensively in the semi-arid tropics of Asia, Africa, and Latin America. Given an extremely narrow genetic base, efforts are being made to develop simple sequence repeat (SSR) markers to provide useful genetic and genomic tools for the peanut research community. A SSR-enriched library to isolate trinucleotide (GGC)n SSRs in peanut was constructed. A total of 143 unique sequences containing (GGC)n repeats were identified. One hundred thirty eight primer pairs were successfully designed at the flanking regions of SSRs. A suitable polymerase was chosen to amplify these GC-rich sequences. Although a low level of polymorphism was... Tipo: Journal article Palavras-chave: Cultivated peanut; Microsatellites; Polymorphism; Simple sequence repeat. Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600005 Registros recuperados: 2 Primeira ... 1 ... Última

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